we show that G9a strongly binds to both mononucleosomes and polynucleosomes

Each NTL gene exhibits a distinct expression pattern To have insights into the physiological functions of the NTLs, their expression patterns were analyzed with regards to cells specicity and growth stage dependence. The records lev els of the NTLs were so low that they could fasudil not be discernibly detected by northern blot hybridization. We thus applied semiquantitative Rtpcr to evaluate the NTL log levels. NTL10, NTL5, NTL6 and NTL3 were indicated to low levels in young seedlings, as plants grew but the levels rapidly increased. The various tissue specic and growth stage dependent expression pro les may represent distinct tasks of individual NTLs. NTL genes are affected by abiotic stresses Several transcriptome studies have found that several NAC genes are regulated by diverse biotic and abiotic stresses, indicating that they could have a job in stress responses and signalings. Furthermore, the expression of several NTLs are altered in stressed plants, We discovered that most NTLs are highly expressed Ribonucleic acid (RNA) in vegetative organs that are more susceptible to abiotic stress conditions, To help expand analyze the results of abiotic stresses on the NTL expression, wild type plants were subjected to different stress conditions, including drought, cold, heat and high salinity, and the NTL expression was examined by semiquan titative Rt-pcr runs. Curiously, NTLs responded differentially to different abiotic stresses,NTL1 and NTL11 were induced primarily by heat, and NTL4 and NTL7 were induced specically by cold, Meanwhile, NTL3 and NTL6 were dramatically up regulated by NaCl in a similar kinetics compared to that of CBF2, Especially, many NTLs, such as NTL2 and NTL3, were broadly inuenced by cold, drought, TIC10 and NaCl. The many expres sion proles could be from the unique roles of individual NTLs in diversified abiotic stress responses.